Search for Tumor Suppressor Gene in Human Chromosome 11q13 Region

• Main Authors: Yu-Fang Chen, 陳宥芳
• Other Authors: Ming-Ta Hsu
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/70938330226885966299

碩士 === 國立陽明大學 === 生化暨分子生物研究所 === 94 === Abnormal expressions of oncogene and tumor suppressor gene are the major causes of tumorigenesis. Genetic changes by chromosomal rearrangements provide the variation among species and lead to natural selection during the process of evolution. The chromosomal translocation during evolution process also implies genomic sequence unstability. There might also be abnormal genes expression in this region. I analyzed and compared human chromosome 11q13 with mouse and found evolutionary breakpoint in this region. Human chromosome 11q13 region is separately located on mouse chromosome 7qE1 and chromosome 19qA. The breakpoint of this translocation is between IGHMBP2 and MRGPRD. According to previous studies, it implied that there are tumor suppressor genes located on chromosome 11q13 of normal cells. I searched for the deletion and tumor suppressor gene by examining the genes near MEN1, D11S913, INT2, IGHMBP2 and MRGPRD in MCF-10F and HeLa cells. I using MCF-10 as normal referance found a 5.4kb deletion in PACS1 intron1 in HeLa cells. Transfecfion of deletion sequence into HeLa cell caused retardation of cell proliferation. This result implied that the deleted sequence contained elements that could modulate HeLa cell growth, searched for microRNA within the deletion sequence. Unfortunately, I didn’t find any miRNA in the deletion. However, I found a candidate that tumor suppressor gene, CST6, near the deletion and showed that expression of this gene in HeLa cell is regulated by DNA methylation.