Effects of viability of lactic acid bacteria on adhesion to Caco-2 and HT-29 cell-line

• Main Authors: Tang-Wen Lu, 盧當文
• Other Authors: Tsong-Rong Yan
• Format: Others
• Language: en_US
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/72617192890957419742

碩士 === 大同大學 === 生物工程學系(所) === 93 === Several studies have reported that non-viable probiotics are able to adhere to tissue culture cells indicating that viability is not necessary for adhesion of probiotics. Other study has been observed that heat and acid treatment of Lactobacillus GG and L. rhamnosus LC-705 enhances the binding capacity of these strains for aflatoxins. Other treatments, such as UV-irradiation, or treatment with alcohol or alkali, did not affect the binding capacity. This suggests that possible changes in the structure of the cell envelope, rather than inactivation of the strains, are responsible for the enhanced binding. During the last years, AFM(atomic force microscopy) study has been observed that there is close relation between adhesion forces of LAB(lactic acid bacteria) and LAB cell wall constituents. This study was to culture several LAB of different adhesion forces, and observed adhesion forces change between normal viable state, nature non-viable state(nature death because no nutrient supplement, reduce risk of change of cell wall constituents) and forced non-viable state(UV-irradiation 24 hours, destroy metabolism function and kept cell wall constituents completely). The result compared different viable state observed that adhesion forces is no obviously change in every strain of LAB adhere to same cell model(Caco-2 or HT-29), and similar adhesion forces trend in every strain of LAB adhere to different cell model(Caco-2 and HT-29), and even bacterial fragment still keep same adhesion density as completely bacteria. Therefore we have proved the adhesion force of LAB has no relation with bacterial viability, but cell wall constituents. We have confirmed the survival of LAB was not important factor on adhesion to human intestine cells.