Summary: | 碩士 === 大同大學 === 生物工程研究所 === 94 === The rosmarinic and ursolic acid content, antioxidant and tyrosinase inhibition activity in Mesona procumbens callus and ten kinds of Lamiaceae plants were examed in this study. The highest content of rosmarinic acid(112.98±0.05 mg/g DW)was obtained from the callus induced from stem explant of Mesona procumbens on MS media supplemented with 4 mg/l NAA or 0.5 mg/l KIN .This result was the same as the wild inflorescence of Solenostemon scutellarioides (red leaf). The contant of rosmarinic acid in cells raised to 129.61±3.24 mg/g DW when cells transfered to the same composition of liquid medium plus 5 g/l PVP. The content of ursolic acid in callus of Mesona procumbens(0.97±0.09 mg/g DW)was less than most other plants of Lamiaceae(1~10 mg/g DW). The comparison of DPPH radical scavenging activity with different organs, inflorescence has the best radical scavenging activity, leaf second and stem third. The DPPH radical scavenging activity of methanol and water extract from callus of Mesona procumbens lower than inflorescence of Lamiaceae, and between the leaf and stem extract. Under 100 μg/ml of extract from callus Mesona procumbens and Lamiaceae plants,the DPPH radical scavenging activity were higher than 90 %. The average of DPPH radical scavenging activity of methanol extract was higher than water extract from all of the tested plants. The highest tyrosinase inhibition activity was expressed in Solenostemon scutellarioides(red leaf). Neither callus nor wild plant of Mesona procumbens had obvious activity of tyrosinase inhibition.
|