| Summary: | 碩士 === 臺北醫學大學 === 牙醫學系 === 94 === Our previous study has shown that pre-injections of 5U botulinum neurotoxin type A (BoNT-A) produces an anti-inflammatory effect on mustard oil injection-induced inflammation. However, the underlying mechanisms of this anti-inflammatory effect are currently unknown. Electrical stimulation of rat’s sciatic nerve was adopted in the present study to induce a pure neurogenic inflammation in rat’s hindpaw. The aims of this study were (1) to investigate the inhibitory effect of BoNT-A on the electrical-stimulation-induced neurogenic inflammation, and (2) to compare the anti-inflammatory effects of BoNT-A, lidocaine (a local anesthetic), omega-conotoxin GVIA (an N-type calcium channel blocker), or L-733060 (an NK-1 receptor antagonist) in order to extrapolate the possible underlying mechanisms of the anti-inflammatory effects of BoNT-A. The results showed that (1) electrical stimulation of sciatic nerve indeed induced significant swelling and plasma extravasation in rat’s hindpaw, (2) BoNT-A, lidocaine, omega-conotoxin GVIA or L-733060 pre-injection significantly reduced (but not completely abolished) the induced swelling, and(3) lidocaine and omega-conotoxin GVIA also significantly reduced the induced plasma extravasation, but BoNT-A and L-733060 did not have such effect. In conclusion, our results have provided direct evidence indicating a partial inhibitory effect of BoNT-A on neurogenic inflammation. However, whether BoNT-A also inhibits the non-neurogenic components of inflammation is unclears.
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