Synthesis of biological active glycoprotein and polysaccharide modification

• Main Authors: Cheng-Hsi Chen, 陳正晞
• Other Authors: 華國媛
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/xf5s75

碩士 === 國立臺北科技大學 === 有機高分子研究所 === 94 === Polysaccharides are important macromolecules with many cellular functions, such as transmitting signals between cells and absorbing waters to maintain moist of tissues. Our project was designed to elicit the functions of neoglycoconjugates. First, polysaccharides were oxidized into aldehyde group. The treatment allowed the sequential reaction, to form covalent bond with 4(4-N-maleimidophenyl) butyric acid hydrazide.HCl.1/2 dioxane. The glycan then was cross linked to BSA, as a protein carrier with maleimide group of 4(4-N-maleimidophenyl) butyric acid hydrazide.HCl.1/2 dioxane. The synthesized compounds were glycoconjugated-BSA. To detect the polysaccharide conjugates, we used lectin blot and periodic acid Schiff (PAS) staining. We then tested the biological functions of polysaccharides. This allowed glycoconjugtates to be taken up by a cell through endocytosis. Glycoconjugate-BSA was able to stimulate immune cells as immuno-modulator. To test the functions of polysaccharides,modification on C-6 hydroxyl group into xanthate and xanthate acetylation. To detect the modified functional group, GC-MS, IR and 1HNMR, 13CNMR were used. We will test the biological functions of these polysaccharides in the future.