The Study of PEG-Salt Aqueous Two Phase Equilibrium and Partition Coefficient of Protein
碩士 === 國立臺北科技大學 === 化學工程所 === 94 === The subject of the present study include the measurement of liquid-liquid equilibrium data and Partition Coefficient of protein in polyethylene glycol –salt –aqueous two phase system. The PEG concentration of liquid-liquid equilibrium data were measured with Refr...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2006
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| Online Access: | http://ndltd.ncl.edu.tw/handle/qu855p |
| Summary: | 碩士 === 國立臺北科技大學 === 化學工程所 === 94 === The subject of the present study include the measurement of liquid-liquid equilibrium data and Partition Coefficient of protein in polyethylene glycol –salt –aqueous two phase system. The PEG concentration of liquid-liquid equilibrium data were measured with Refractometer and the salt concentration were measured with Atomic Absorption spectrophotometer. The polymer of glycol was PEG4000,and the salts were sodium sulfate、sodium bisulfate and sodium chloride. Experiment temperatures were chosen at 15℃、20℃、30℃.pressure in normal. The Partition Coefficient of protein was measured with PEG4000- sodium sulfate–aqueous two phase system, protein was Albumin form chicken egg Experiment temperatures were chosen at 15℃、20℃、30℃.pressure in normal.
According to experimental data, the phase behavior of sodium sulfate and sodium bisulfate system depend on temperature. Sodium chloride system can not spread to two phase. The Partition Coefficient of protein was increase with the tie line length. All of experimental data will correlate by Extended UNIQUAC model.
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