| Summary: | 碩士 === 亞洲大學 === 生物資訊研究所 === 94 === Natural antisense transcripts (NATs) were first described in prokaryotes, and also were found in the eukaryotic cell later. The ‘antisense’ term includes transcripts containing long sequences complementary to sense transcripts, and the ‘nature’ term means endogenous. This transcriptome were found to regulate the expression of sense transcripts involved in diverse biological function, such as transcriptional interference, RNA masking, double-stranded RNA (dsRNA)-dependent mechanisms, genomic imprinting, alternative splicing, X-inactivation. In the human genome, it was predicted about 1% to 20% of the human genes were influenced by antisense transcripts.
The antisense had been widely used as drug design application. But there is no literature confirms the relationship between the tumorgenesis and NATs. Our laboratory had recently cluster the sense-antisense gene pairs, based on NCBI (National Center for Biotechnology information) expressed sequence tags source and NCI-CGAP (National Cancer Institute – Cancer Genome Anatomy Project) annotation. The dataset identify 6,551 NATs clusters. We random selected three NATs gene pairs and performed Q-PCR (real-time quantitative polymerase chain reaction) examination. The mRNA expression level of NATs shows different expression between human cancer and normal-tissue. It proved the reliable of our dataset and that the NATs do play a role in the tumorgenesis.
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