Study on the mechanism of bitter gourd combined with spirulina improved high fat diet-induced insulin dependent on genetically deficient TypeⅡ diabetic mice

• Main Authors: Hsun-Hsin Yu, 游嵩勳
• Other Authors: Jiunn-Jye Chuu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/87505800919541810032

碩士 === 南台科技大學 === 生物科技系 === 94 === Type 2 diabetes mellitus (non-insulin-dependent diabetes meilitus, NIDDM) diabetes mellitus was expressed with increasing insulin resistance, eventually results in beta-cell dysfunction. Currently, the bitter gourd extract (Momordica charantia), spirulina and bitter gourd combined with spirulina has been studied for diabetes for lowering blood glucose levels in patients with diabetes mellitus and its complications (eg. nephropathy, cataract, insulin resistance). In order to elucidate the assessment of blood glucose, triglyceride, cholesterol and oral glucose/insulin tolerance after bitter gourd extract, spirulina and bitter gourd combined with spirulina oral administration at doses of 200 mg/kg/day for 8 weeks, this diabetic KK/HIJ mice model with high fed diet-induced hyperglycemia diabetes will be provided. Finally, the involvement of GLUT-4, IRS-1 and PPAR-γ expression on several metabolic organs of diabetic mice by western blotting will be manipulated. Our preliminary data indicated that some induced-hyperglycemia KK-mice (over 150mg/dl, within fasting period) show abnormal triglyceride/cholesterol increase, glucose tolerance prolongation (OGTT) and decrease of GLUT-4 expression while the insulin maintained normal level by insulin ELISA test. To compare the similar type 2 diabetes KK-mice model, a part of STZ induced diabetes mice were grouped (blood glucose >100 and <200mg/dl) as type 2 diabetes mice model (normal insulin level), we found that the bitter gourd combined with spirulina also improve the glucose tolerance and reduce triglyceride/cholesterol level with increasing GLUT-4 , IRS-1 and PPAR-γ expression skeletal muscle,liver and adipocyte of high fat diet -induced diabetic mice by western blotting. Lately, the SRB assay and flow cytometry will claim the cytotoxicity and apoptotic index, respectively in pancreatic β cell line in vitro in a concentration-dependent manner.