Effect Investigation of Scaffold Porosity Size on Cell Growth for Tissue Engineering Using Rapid Prototyping Technology

• Main Authors: Hsu Fang-Hao, 許芳豪
• Other Authors: Jeng-ywan Jeng
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/3gzc8j

碩士 === 國立臺灣科技大學 === 機械工程系 === 94 === Tissue Engineering scaffold is a porous scaffold , it can offer cell an excellent environment to adhesion , grow , differentiate and form ECM , Documents point out that the best porosity of the bone regeneration is under 350μm , but still unable to learn that the influence of porosity size upon cell growth in this range . Therefore , this research is direct to the best porosity suitable for growth range of the bone regeneration , utilize rapid prototyping technology . Layered manufacturing techniques . Fabricating porous scaffolds of PCL and the width of fiber is about 130~160 μm . Therefore , select the 200 , 250 , 300μm of line diameter to fabricate scaffolds , and porosity sizes are between 70~180μm . To implant and culture the osteoblast cell (MG63) in the scaffold afterwards. Know that cells are the best activity for the scaffold of line diameter of 200 μm porosity by MTT. On the test of every physical property and mechanical property fabrication of PCL scaffold . Know that PCL is dissolved with the organic solvent chloroform . Fabricating the scaffold material will not change nature property of PCL and fabricate the scaffold material has good mechanical intensity . Therefore , this experiment can be consulted as the experiment that implant scaffold in animal's body in the future . Direct to the fabrication of PCL scaffold of Tissue Engineering , it appears milkiness wholly and does not have opaque quality . Therefore , it can not observe the cell growth immediately with the microscope after culture cell . Then we use the agar as material , through the advantage of transparent quality to build the Tissue Engineering scaffold , by means of this to improve above-mentioned shortcomings .