| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 94 === Rice bran oil (RBO) is an inexpensive raw material for the production of biodiesel. Utilization of by-product such as defatted rice bran for the production of proteins, carbohydrates, phytochemical, and the isolation and purification of value-added nutraceuticals generated, such as ��-oryzanol and tocos, during biodiesel production from RBO are attractive options to lower the cost of biodiesel.
Crude RBO contains 1.8~3.0% ��-oryzanol. ��-Oryzanol has important applications in food, cosmetic and pharmaceutical industry. The object of this investigation is to isolate ��-oryzanol from residue obtained during the production of biodiesel from RBO. Using RBO as the starting substrate, the content of ��-oryzanol could be raised to 16.18 % (recovery 83.62%) by a series of steps which included degumming, acid-catalyzed esterification and vacuum distillation (3.4~3.6×10-2 torr, 192oC). In the distillation step, more than 95% of low-boiling point components, such as free fatty acid (FFA) and fatty acid methyl ester (FAME) could be distilled. After distillation, diglyceride and triglyceride in the residue were converted into FAME by enzyme (Novozyme 435) catalyzed esterification. The organic phase of the reaction product was then subjected to silica gel column chromatography. After collecting the ���{oryzanol enriched fraction and removing the solvent, the content of ��-oryzanol could be raised from 16.18 % to 73.75 % with a recovery of 79.75% (total recovery 66.88%).
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