| Summary: | 碩士 === 國立臺灣大學 === 臨床牙醫學研究所 === 94 === Background and purpose: Previous studies have shown a significant association of high-risk human papillomavirus (HPV) types with cervical and oral squamous cell carcinomas. However, the etiologic role of HPV in the development of oral verrucous carcinoma (OVC) is still unclear. In this study, we tried to elucidate whether there was a close relationship between all or high-risk HPV types and OVCs.
Methods: DNA samples were purified from 75 OVC specimens and 30 normal oral mucosa (NOM) specimens. A nested polymerase chain reaction (PCR) was used to amplify the consensus L1 region of the HPV genome in DNA samples using two sets of HPV primers (MY09/GP6+ and GP5+/GP6+). This procedure combined with a gene chip HPV typing and DNA sequencing was able to detect 39 HPV subtypes in our samples.
Results: We found no significant difference in the overall HPV-positive rate between OVC samples (15%, 11/75) and NOM samples (20%, 6/30, P>0.05) as well as between OH-associated OVC samples (14%, 10/71) and non-OH-associated OVC samples (25%, 1/4, P>0.05). In addition, there was also no significant difference in the positive rate of high-risk, low-risk or unclear-risk HPV types between OVC samples (4%, 11% or 3%, respectively) and NOM samples (17%, 3% or 0%, respectively, P>0.05) as well as between OH-associated OVC samples (3%, 11% or 3%, respectively) and non-OH-associated OVC samples (25%, 0% or 0%, respectively, P>0.05). However, HPV type 16 and multiple HPV types were detected more frequently in NOM samples (17% and 13%, respectively) than in OVC samples (0% and 1%, respectively, P<0.05). In 11 HPV-positive OVC samples, low-risk types (11, 72 and 74) were found more frequently (73%, 8/11) than high-risk types (18 and 31; 27%, 3/11) or unclear-risk types (53 and 62; 18%, 2/11), but the difference was not significant (P>0.05).
Conclusion: The relatively low overall and high-risk HPV-positive rates in our OVC samples, especially in OH-associated OVC samples, compared to those in NOM samples suggest that there is no significant association of all and high-risk HPV types with OVCs, especially OH-associated OVC.
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