Polymer Surface Modification Effect of Platelet Storage Condition Research

• Main Authors: Hung-Hsing Lin, 林弘欣
• Other Authors: Tai-Horng Young
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/04026735003876506650

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 94 === We fabricated poly(vinyl chloride) 、poly(vinyl alcohol) and poly(ethylene-co-vinyl alcohol) polymer membranes by the method of dry processing. Diamine was grafted on the PVC membrane surface by the chemical reaction with the chloride groups. Diisocyanate was grafted on EAVL and PVA membrane surfaces by chemical reaction with the hydroxyl groups. Afterwards, amino group were grafted on these membranes. Also, we analyzed the surface properties of these membranes by means of Micro-ATR、Orange II salt and contact angles. To investigate the platelet storage condition and its qualities with diff. polymer membranes and L-Arginine effects, we used the cell viability method；MTT and LDH、platelet aggregation test、platelet surface marker labeling assay with flow cytomerty, clouding P-Selectin、GpIIb-IIIa complex and Survival-Apoptosis ratio, and platelet adhesion and shape change using take SEM picture. Post-storage platelet were shown the less adhesion and better qualities maintain on the modified membranes, we believed that course with the modified membrane were to provide more hydrophilic condition that can inhibit platelet adhesive on the polymer membranes. When we used L-Arginine to regard as the storage addition solution, we can get more better results on each experimental groups. But L-Arginine effect were lost after 3days. In order to solve this problem, we designed the membrane；EVAL grafting the L-Arginine on the membrane surface, the experiment result were shown the most less platelet adhesion number and best qualities maintain. So, we believe the membrane modification and additive L-Arginine solution to regard as addition solution can effectively improve platelet storage condition and its storage qualities.