| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 94 === We fabricated poly(vinyl chloride) 、poly(vinyl alcohol) and
poly(ethylene-co-vinyl alcohol) polymer membranes by the method of
dry processing. Diamine was grafted on the PVC membrane surface by
the chemical reaction with the chloride groups. Diisocyanate was grafted
on EAVL and PVA membrane surfaces by chemical reaction with the
hydroxyl groups. Afterwards, amino group were grafted on these
membranes. Also, we analyzed the surface properties of these membranes
by means of Micro-ATR、Orange II salt and contact angles.
To investigate the platelet storage condition and its qualities with diff.
polymer membranes and L-Arginine effects, we used the cell viability
method；MTT and LDH、platelet aggregation test、platelet surface marker
labeling assay with flow cytomerty, clouding P-Selectin、GpIIb-IIIa
complex and Survival-Apoptosis ratio, and platelet adhesion and shape
change using take SEM picture.
Post-storage platelet were shown the less adhesion and better
qualities maintain on the modified membranes, we believed that course
with the modified membrane were to provide more hydrophilic condition
that can inhibit platelet adhesive on the polymer membranes. When we
used L-Arginine to regard as the storage addition solution, we can get
more better results on each experimental groups. But L-Arginine effect
were lost after 3days.
In order to solve this problem, we designed the membrane；EVAL
grafting the L-Arginine on the membrane surface, the experiment result
were shown the most less platelet adhesion number and best qualities
maintain. So, we believe the membrane modification and additive
L-Arginine solution to regard as addition solution can effectively improve
platelet storage condition and its storage qualities.
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