Using Acid and Alkali Pretreatment (rice hulls) to Enhance Cellulase Hydrolysis Efficiency

• Main Authors: Wei-Fan Huang, 黃維凡
• Other Authors: 謝志誠
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/04172032116486054624

碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 94 === Rice hull, a complex lignocellulosic material with high lignin and ash content, has the potential to serve as a low-cost feedstock for production of ethanol. In this study, rice hull was pretreated by dilute NaOH and H2SO4 at different temperature (30, 60, 90°C) with different concentration(0.25, 0.2, 0.15 M) and different treatment time（20、40、60 minutes）. Reducing sugars in the hydrolyzate are analyzed. The solid residues were hydrolyzed by cellulase from Trichoderma reesei C2730 under conditions of pH(4.6), temperature (50℃), enzyme concentration (21U/mL) and enzyme to substrate ratio (1%). The effects of pretreatment time, temperature and acid/base concentration on enzymatic hydrolysis are studied. The effect of acid pretreatment is not obvious. Results show that alkaline pretreatment can enhance the saccharification. Pretreated rice hull hydrolyzed with Trichoderma reesei C2730 cellulase showed 80% sacchrification. Keywords: ethanol; acid pretreatment; alkaline pretreatment; rice hull; enzymatic hydrolysis