Proteomic Research and Volatile Compounds Analysis of Tea Leaves with Feeding Damage by Jacobiasca formosana Paoli

• Main Authors: Kuan-Li Kuo, 郭冠黎
• Other Authors: 李昆達
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/62548716149149030021

碩士 === 國立臺灣大學 === 微生物與生化學研究所 === 94 === Pong-Fong tea, also named Oriental Beauty tea, a unique tea only produced in Taiwan, is famous for the honey-like and ripen-fruit-like aroma. To use the tea leaves infested by a kind of small tea insect, smaller green leafhopper (Jacobiasca formosana Paoli), as the tea making material is the characteristic in production of Pong-Fong tea. Therefore, the feeding damage on the tea leaves is thought the key effect to the aroma formation of Pong-Fong tea. In the proteomic analysis, the elevated expression of four proteins was observed, including a heat shock protein, a superoxide dismutase, a glyoxalase, and a thiosulfate sulfurtransferase, which has been reported to be involved in plant stress or defense reactions. Through the volatile compounds analysis by GC/MS, we found that tea leaves with feeding damage by J. formosana Paoli contained more benzaldehyde, 3-carene, linalool, linalool oxides, linalool derivatives, benzyl nitrile, indole, alpha-farnesene, methyl salicylate, benzoic acid and cis-jasmone. It is concluded that the feeding damage in tea leaves by the phloem feeding insect, J. formosana Paoli, resulted to their stress and defense response, and the volatile compounds were emitted probably for signaling, herbivore deterrence and attraction for the predators or parasitoids of the herbivore.