Promoter Activity Analyses of Banana Polyubiquitin and beta-1,3-Glucanase genes

• Main Authors: Yung-Chen Lin, 林運辰
• Other Authors: Yi-Yin Do
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/36183669071388299968

碩士 === 國立臺灣大學 === 園藝學研究所 === 94 === To analyze promoter activity of banana polyubiquitin Mh-UBQ3, Mh-UBQ4 and beta-1,3-glucanase Mh-BGL1 genes, 5’-flanking region upstream translation start codon of each gene was constructed into stable transformation vector to drive reporter gene β-glucuronidase (GUS). Then, these expression vectors were transformed into Arabidopsis and tobacco. Results of GUS histochemical analysis for both of Arabidopsis and tobacco transgenic plants reveals that each promoter expressed differentially in tissues and development stages. No detactable expression activity was observed in Mh-UBQ3::GUS transgenic plants for both Arabidopsis and tobacco. In Mh-UBQ4::GUS transgenic plants, expression pattern in cotyledon was greatly affected during seedling development, while it always had strong expression activity in the newly born leaves. GUS expression in Mh-BGL1::GUS transgenic plants was tissue and developmental specific. When cotyledons was encloed, expression in the junction site of cotyledons and hypocotyls was weak. As it expanded, expression activity was gradually eleveated. Result of treatment analysis indicated that expression activity of Mh-UBQ1 gene promoter could be induced by plant growth regulators IAA, BA, ABA, GA3, 2,4-D, SA, and Me-JA and by abiotic stimuli such as drought and high salt. Expression activity of Mh-UBQ2 gene promoter could be induced by palnt growth regulators such as IAA, BA, ABA, GA3, 2,4-D, SA, and Me-JA and by abiotic stimuli like high salt, wounding, cold and dark, and heat. Expression activity of Mh-UBQ3 gene promoter was not induced by any treatment in the experiment. Expression activity of Mh-UBQ4 gene promoter could be induced by plant growth regulators SA and ethylene and by abiotic stimule like dark and heat while repressed by ABA and Me-JA. Promoter activity of Mh-BGL1could be indeuced by GA3 and ethylene but repressed by IAA, BA, ABA, 2,4-D, SA, and Me-JA.