Inspection of cellular and molecular level in Bambusa oldhamii and Dendrocalamus Iatiflorus Munro albinisms

• Main Authors: Fu-Hui Wu, 吳馥卉
• Other Authors: Wei-Chin Chang
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/72115494360283778886

碩士 === 國立臺灣大學 === 園藝學研究所 === 94 === Building the system of bamboo tissue culture reproduction could propagate and provide for virus-free bamboo plantlet. But propagating bamboo plantlet in tissue culyure, there were producing albinisms in subculture. Cause of many species albino mutant in tissue culture regenerating from single-cell microspore culture was chloroplast genome deletion. But the cause of bamboo albinism through nodal bub culture prorpagating was not published. The object of this study was capitalized bamboo somatic cell mutants and using polymerase chain reaction, reverse transcription polymerase chain reaction and microarray technology to investigate albinisms. psaB (PSI P700 apoprotein A2) DNA was 17% compare with wild type among the 8 genes that copy numbers decreased in in Dendrocalamus Iatiflorus Munro albino mutant. There was no gene copies number decreased of 31 chloroplast genomic genes in Bambusa oldhamii albinism but 6 genes expression has gone down. In addition, using microarray has screened genes expression decreased obviously in Bambusa oldhamii three albinism mutamt. The cause of albinisms were difference between Bambusa oldhamii and other tissue culture system, but the mechanism was unclear in present.