Inhibitory mechanisms of the ethanolic extract of mycelia of Antrodia cinnamomea on the growth of human lung cancer and hepatoma cells

• Main Authors: Hui-Chuan Yu, 游慧娟
• Other Authors: Lee-Yan Sheen
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/64681448829386987432

碩士 === 國立臺灣大學 === 食品科技研究所 === 94 === Antraodia cinnamomea is well-known as a traditional Chinese medicine in Taiwan. Because the growth rate of the natural A. cinnamomea in the wild is very slow, it is difficult to be cultured in green house. It is very expensive to obtain to fruiting bodies. Additionally, it might be a possible and economical way to utilise the submerged fermentation to produce the product of A. cinnamonea with stronger bioactivity. Previous researches have shown A. cinnamomea has many biological activities, such as anti-cancer activity, hepatoprotection, anti-oxidation, anti-inflammation, neuroprotection, and so on. In addition, lung and liver cancer are very common malignant tumors in Taiwan. This study was to investigate the effect of ethanolic extract of mycelia of A. cinnamomea (EMA) in submerged fermentation on the human lung (A549) and liver (SK Hep-1 and HA 22T/VGH) cancer cell lines and their possible mechanisms. The results showed that EMA inhibit the cell viability of A549, SK Hep-1 (p53-positive) and HA 22T/VGH (p53-deletion, HBsAg [+]) for 48hr, and their IC50 value were 30.9、48.2 and 117.1 μg/ml, respectively. The results of cell cycle analysis showed that EMA induced cell cycle arrest in S (A549, 40 μg/ml), G0/G1 (SK Hep-1, 20 μg/ml) and S (HA 22T/VGH, 160 μg/ml) phase. Furthermore, EMA could induce apoptosis (accumulation of sub G1 phase) in A549 (80 μg/ml), SK Hep-1 (80 μg/ml) and HA 22T/VGH (160 μg/ml), and the DNA ladder were presented in these three cell lines by DNA electrophoresis. By fluorescence microscope, results indicate EMA induced A549, SK Hep-1 and HA 22T/VGH apoptosis through induction of caspases-3 and expression caspase-3.