Intravital Multiphoton Microscopy of Hepatic Metabolism

• Main Authors: Yuan Liu, 劉源
• Other Authors: Chen-Yuan Dong
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/85322916428156842584

碩士 === 國立臺灣大學 === 物理研究所 === 94 === Multiphoton microscopy is an advanced nonlinear optical technique developed in early 1990s. Including two-photon fluorescence and second harmonic generation, its optical sectioning ability and minimally invasiveness makes it a preferable choice for intravital observation. In vivo and real-time imaging of liver is vital for elucidating hepatic dynamics. Here we develop a novel chronic window device and surgical methods to investigate hepatic dynamics using multiphoton microscopy. The hepatic metabolism is visualized by imaging sequential uptake of 6-carboxyfluorescein diacetate from hepatocytes and its excretion into bile canaliculi. The excretory process is then employed to evaluate the function of liver of cholestasis, which is established by ligating the common bile duct. The liver with bile duct ligated for 1 or 2 days shows a relatively slow rate of excretion compared to the normal liver. In this work, we demonstrate the potential of intravital multiphoton microscopy of hepatic dynamic processes. By these techniques, various liver diseases and mechanisms can be explored in the future.