Fundamentals and Applications of molecular recognition using 3, 4, 5, 6-Tetrahydro-bis-(pyrido [3, 2-g]indolo)[2, 3-a: 3’,2’–j]acridine as host molecule

• Main Authors: Yu-Shan Yeh, 葉育姍
• Other Authors: 周必泰
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/06625317903560727969

碩士 === 國立臺灣大學 === 化學研究所 === 94 === Abstract The concept of molecular recognition has been applied extensively in the field of biochemistry and many other fields. Here, designed and synthesized cleft-like host molecule, which mimics protein structure and its binding abilities, is used in binding with biomolecules such as urea and glucopyranosides. Spectroscopic methods act as powerful tool in discriminating between free and complexed molecules and evaluating their association entities quantitatively. This thesis is organized into four chapters. We start with a brief introduction of the concept of molecular recognition. Some basic principles and experimental setups of steady-state spectrometry, the general errors and correction methods are discussed in chapter 2. In the two successive chapters, utilizing excited state proton transfer reaction, 3, 4, 5, 6-Tetrahydro-bis-(pyrido[3, 2-g]indolo)[2,3-a:3’, 2’–j]acridine acts as an ideal host molecule to bind with different biomolecules efficiently in both protic and aprotic solvents. High sensitivity and selectivity are achieved via fluorescent detection method, and the association constants are deduced accordingly. In addition, to accomplish chiral recognition, circular dichroism (CD), fluorescence-detected circular dichroism (FDCD) and theoretical approach are performed in differentiation of optical isomers. The extension of these applications into aqueous environments is still the topic of endeavor.