| Summary: | 碩士 === 國立臺灣海洋大學 === 海洋生物研究所 === 94 === Anisakis simplex is a parasite in marine fishes and mammalian, and may infect human being to induce Anisakiasis through seafood . The purpose of the work was to investigate the prevalence, mean intensity and abundance of the parasites (Anisakis spp.) in Trichiurus lepturus, Scomber japonicus and Trachurus japonica in the ocean around Keelung isle, Taiwan. By using light microscopy, scanning electron microscopy and molecular biotechnology, the biology of A. simplex and the effect of gamma-irradiation on the parasite control were studied. The prevalence of the parasite in T. lepturus, S. japonicus and T. japonica was 91%(66/72), 39%(28/72) and 89%(64/72), respectively. The mean intensity of the parasite in T. lepturus, S. japonicus and T. japonica was 39, 2 and 62, respectively. The abundance of the parasite in T. lepturus, S. japonicus and T. japonica was 62, 3 and 80, respectively. With PCR - based restriction fragment length polymorphism method and phylogenetic analysis, Anisakis spp. in the ocean around Keelung isle were separated two groups. Gamma-irradiation (5 and 10 kGy) offered a good control on the parasites. A. simplex with 10 kGy gamma-irradiation showed skin injuries. However, the nutrition of fishes irradiated was not significantly changed. Irradiation is a good option for controlling Anisakis spp. although the biology of the parasites needs further studies.
|
|---|
