| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 94 === Abstract
Antrodia cinnamomun (Polyporaceac, Aphyllophorales) is a well-known Chinese herbal medicine in Taiwan. It has been using for the treatment of a variety of diseases, such as food and drug intoxication, diarrhea, abdominal pain, hypertension, itchy skin and liver cancer. At present, it has been known that Antrodia cinnamomun can play the role on anti-oxidation, anti-inflammation, hepatoprotective and antitumor. The purpose of this study is to evaluate the effect of ethanolic extracts of mycelia cultured from the biologically active fraction of A. cinnamomun on the inhibition of cell viability and its cytotoxic induced mechanism in hepatoma cell line (Hep G2). In the research, Hep G2 is uesed for testing cell viability to evaluate the active fraction of ethanolic extracts of mycelia cultured from the biologically active fraction of A. cinnamomun. And the active fraction J101 is picked out to probe the mechanism of apoptosis. The IC50 value was 0.5�慊/ml when being treated HepG2 cell line with J101 for 24 and 48hrs. By testing DNA fragment , it was known that J101 leads the cell death was by apoptosis. We nearly explore to the mechanism of apoptosis that the more the dose of caspase 3 enzymes activity that behave. The present result implies that ethanolic extract from A. cinnamomun mycelia may lead to inhibit proliferation and induces apoptosis pathway on hepatoma HepG2 cell line. To further exploration of the TRAIL resistance in hepatoma cell line, we process cells with TRAIL and EACPS. It is founded that induce Hep G2 and Hep 3B apoptosis. Therefore, according to the result of this study, we estimate that ethanolic extracts of mycelia cultured from A. cinnamomun of biologically active compounds can contribute to apoptosis of Hep G2 which is triggered by activating caspase 3 enzyme .
Key words: Antrodia cinnamomun, liver cancer, apoptosis, caspase, TRAIL.
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