Effect of reduced water on carbohydrate and lipid metabolism in streptozotocin-induced type 2 diabetic rats

• Main Authors: Ying-Chiun Chao, 趙盈鈞
• Other Authors: Meng-Tsan Chiang
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/00412569107119993894

碩士 === 國立臺灣海洋大學 === 食品科學系 === 94 === Abstract Oxidative stress has been suggested to be a contributory factor in development and complication of diabetes. In the present study, we investigated the effect of reduced water (RW), a cathodic solution produced by the electrolysis of tap water, on carbohydrate and lipid metabolism in streptozotocin–nicotinamide induced diabetic rats. Male Wistar rats were randomly divided into four groups: (1) rats drink water; (2) rats drink RW; (3) diabetic rats drink water; (4) diabetic rats drink RW. All groups fed with high fat diet and water ad libitum. This experiment was done for 6 weeks. In normal rats groups, rats drank RW had a significant decrease in blood glucose, triglyceride, cholesterol, insulin, leptin concentrations and TBARS value and a significant increase in plasma GOT level. The ability of RW to decrease the blood glucose may be correlated with an increase in hepatic hexokinase activity and lower both proximal and distal region intestinal sucrase specific activities. Although no significant difference in fecal pH value was observed, RW induced an elevated fecal mucinase activity in normal rats. In addition, drinking RW caused a significant increase in the activities of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase, and a significant decrease in glutathione concentration in liver of normal rats, but there were no significant differences in hepatic TBARS value and uncoupling protein-2 protein expression. Also, rats drank RW had a significant increase in kidney/100 g B.W. but a decreased in creatinine levels were observed. As to diabetic rats groups, however, only a slightly significant decrease in plasma glucose and glucose area were observed. In additional, lower hepatic glucose-6-phosphatase and distal region intestinal disaccharidases specific activities were observed in diabetes rats drank RW than those drank water. In hepatic antioxidant status, a significant increase in GSH concentration was observed in diabetic rats drank RW. These results indicate that RW may regulate the plasma glucose and lipid in normal rats, but has limited efficient in diabetic rats.