Effects of yam (Dioscorea alata L.) mucoploysaccharide on human skin fibroblasts proliferation, Migration, and extracellular matrix production.

• Main Authors: Pei-Ching Yu, 余珮菁
• Other Authors: Rong-Huei, Chen
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/60458365445831493589

碩士 === 國立臺灣海洋大學 === 食品科學系 === 94 === The purpose of this study is to explore the effects of yam mucopolysaccharide from Dioscorea alata L. on wound healing. The thesis includes three parts : 1. The effects of yam mucopolysaccharide on human skin fibroblasts proliferation in vitro. 2. The effects of yam mucopolysaccharide on human skin fibroblasts migration in vitro. 3. The effects of yam mucopolysaccharide on the extracellular matrices production by the fibroblasts in vitro. The results obtained might shed the light of the effects of yam mucopolysaccharide on skin wound healing. The results show that yam mucopolysaccharide enhanced human skin fibroblasts proliferation. The higher the concentrations of yam mucopolysaccharide (ranged between 1.56~25 μg/ ml) in 10% serum medium, the better the results on the skin fibroblasts proliferation. The effects of yam mucopolysaccharide on fibroblasts proliferation are more significant for the skin fibroblasts cultured in 1% serum medium at concentrations range between 0.39~200μg/ ml. The enhancement is dose-dependent between 0.39~100μg/ ml on 1% serum medium. The optimal concentration is 100μg/ ml. Yam mucopolysaccharide can also promote migration of human skin fibroblasts. The cells in 200 × times microscopic fields were counted. The beneficial effect of yam mucopolysaccharide on skin fibroblasts migration was dose-dependent. The effects of yam mucopolysaccharide on the fibroblasts migration is significantly different from the control when concentrations are 6.25 μg/ ml and 50 μg/ ml. The results of the effects of yam mucopolysaccharide on the extracellular matrices excretion by the fibroblasts, showed that yam mucopolysaccharide facilitated fibroblasts to secrete either collagen or fibronectin. At a concentration of 12.5μg/ ml of yam mucopolysaccharide, the collagen secretion by the skin fibroblasts is significantly higher than the control. So as the enhancement on the secretion of fibronectin at the slightly higher concentrations (over 12.5μg/ ml).