| Summary: | 碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 94 === The zebrafish has emerged as an excellent model organism for study. External development and optical clarity during embryogenesis allow for visual analyses of early developmental processes, and high fecundity and short generation times facilitate genetic analyses. The gene function way is similar to mammal and the organ forms of gene control and embryo's development is similar to other vertebrates. Tetracycline-controlled system (Tet-on and Tet-off) that could be operated gene translate turn-on or turn-off by time specific and highly tissue-specific way. So we can make use of Tet-on mechanism to establish have tissue-specific and can be induced transgenic zebrafish system. I-FABP (intestinal fatty acid binding protein) is the specific protein in the intestine. Utilizing the I-FABP promoter to make the specific gene express in the intestines but will not express in other position. Bax gene is the pro-apoptotic gene that can cause the cell apoptosis. In zebrafish we can observe the phenomenon displayed by microscope. In this experiment, we utilize Tet-on system, I-FABP promoter and bax genes to establish this system. In this system, bax gene only expressed in the intestines and at usual bax gene was suppressed and induced by dox to cause cells apoptosis. We can use this system to analyze intestinal development of transgenic zebrafish. We used microscope, Genomic DNA, RT-PCR analyze to confirm the establishment of this Tet-on system.
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