Wnt4、Follistatin and Fig α in the Female Spotted Grouper (Epinephelus coioides ) to

• Main Authors: Guan-Yun Peng, 彭冠運
• Other Authors: Ching-Fong Chang
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/44637444084154856516

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 94 === Abstract There are two types of sex differentiation in fish. One is gonochorism, and the other is hermaphroditism. Hermaphroditism also can be separated into protogyny and protandry, and spotted grouper (Epinephelus coiodies) is belonged to protogyny. Grouper will differentiate to monosex female in the first year after hatching and sex change to male after several years. The development of monosex female in grouper provides as an unique model to study female sex differentiation. The objectives were to investigate the possible roles of Fig α, follistatin, and Wnt4 in the female sex differentiation of spotted grouper. According to the histological data, gonad differentiation of grouper was occurred at 150 dah in spotted grouper. And in the analysis of gene expression, high levels of Wnt4 transcripts were found at 150 dah, follistatin transcripts were increased at 150 dah and 195 dah, Fig α and VASA were appeared at 150 dah and increased progressively. In order to further studyed the regulation of follistatin by estrogen, we fed grouper with aromatase inhibitor. In the analysis of gene expression, Wnt4 was suppressed by the treatment of aromatase inhibitor. Follistatin transcripts were stimulated after one month of aromatase inhibitor treatment. Fig α was not affected by aromatase inhibitor. In conclusion, Wnt4 and follistatin might be important to female sex differentiation and also could be up-negative by estradiol. Fig α was suggested to be important in oocyte differentiation and development but not affected by estradiol.