Investigation of the correlation between OTEX and prostate cancer

• Main Authors: Yu-lin Ho, 何雨霖
• Other Authors: Hsiu Mei Hsieh-Li
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/83982066744346772784

碩士 === 國立臺灣師範大學 === 生命科學研究所 === 94 === English Abstract OTEX gene is localized on the human X chromosome and expressed in testis, epididymis, prostate, ovary and brain. No ortholog of OTEX gene has been identified in mouse genome through intensive GenBank search and cDNA library screening. Zoo blot analysis reveals that OTEX is only present in human genome which suggests that OTEX appeared in a later period during evolution, and may be involved in an important genetic regulation of the complicated human physiology. OTEX expression was also identified in LNCaP prostate cancer (PCa) cell line, and the expression was stimulated dramatically following androgen treatment. Real-time PCR analysis was thus used to quantitate the expression levels of OTEX and PSA in BPH and PCa patients. OTEX gene was transfected into PCa cell line, PC3, to further uncover the functional significance of OTEX. An inducible cell line is established and used to investigate the influence of the physiologic significance of OTEX gene on PC3 cell line. Promoter scan was used to search binding motif of upstream and intron 1 of OTEX gene, and an putative androgen response element (ARE) site was identified in the upstream about 1 kb region. To test whether OTEX is under the regulation of androgen directly, OTEX promoters with or without the putative ARE were analyzed by reporter assay. Results obtained from these studies should help in uncovering the correlation between OTEX gene and PCa.