Development of on-line PVC packed micro-column ICP-MS hyphenated system for the determination of trace elements in saline sample

• Main Author: 李字為
• Other Authors: 孫毓璋
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/89085689293793039791

碩士 === 國立清華大學 === 生醫工程與環境科學系 === 94 === Owing to trace analytes and high matrix content are always the main obstacles should be overcome in conjuncting analytical chemistry with environmental and biological science, the development a convenient and robust analytical technique dealing with high salt contented samples is also thought crucial for the determination of trace metals in saline sample. Nowadays, solid phase extraction is the most popular method for the preconcentration of trace elements. However, the complicated operation procedure and the use of large volume of chemicals can always lead to server contamination problem. For purpose of simplifying the analytical procedure and minimizing the volume of used chemicals, a solid phase extraction method, based on the polymer-metal interaction, was developed to couple with on line ICP-MS measurement. In this study, the potential of a micro column packed with polyvinylchloride (PVC) beads was ensured for the construction of a SI-lab on valve (SI-LOV) on-line matrix removal and metal preconcentration system. Based on optimized results, the performance of PVC packed-column for the preconcentration of trace Mn, Co, Ni, Cu, Zn, Cd and Pb from aqueous samples was mainly depending on pH value , sample flow rate and eluent flow rate. By the use of SI-LOV and automization techniques, the overall procedure can be accomplished within 8 min and the detection limits of analyte ions are in the range of ng/L to sub-ng/L. The accuracy of the developed method was confirmed by the analysis of certified reference materials of seawater and urine samples.