| Summary: | 碩士 === 國立清華大學 === 化學系 === 94 === In 1936, Gordon L. Locher formulated his binary concept of treating cancer. In particular, there exist the possibilities of introducing small quantities of strong neutron absorbers into the region where it is desired
to liberate ionization energy (a simple illustration would be the injection of a soluble, nontoxic compound of boron, gadolinium, followed by bombardment with thermal neutron).The efficacy is dependent on the cross-section of neutron capture element (1 barn = 10-24cm2). The 10B isotope has a neutron capture cross-section of 3838 barn and the 157Gd has a neutron capture cross-section of 255000barn. There are two important factors that it can’t be solved in trail now: (1) target delivery of drugs to tumor sites; (2) sufficient high dose at tumor sites as predicted by theoretical calculation.In the literature, no one has ever reported the use of carbon nanoparticles to carry a large quantity of 10B or 157Gd element for cancer therapy.The thesis was divided into two parts:
(a)First one We prepare boron or gadolinium filled magnetic carbon nanoparticle by DC arc discharge method. Carbon nanoparticles surface-grafted with polyacrylic acids were dissolved in H2O, and then further modified with folic acid. The folic acid moiety can drive carbon nanoparticles to the folate receptor sites on the surface of tumor cells. Second one is to focus on cell culture、MTT assay、toxicity assay and neutron irradiate cell with or without HeLa cell.
We use many spectroscopic methods such as, (TEM、SEM、XRD、EELS mapping and ICP-AES…..etc) to determine the presence and the quantity of boron or Gadolinium in carbon nanoparticle. After carrying out toxicity assay, we evaluate the dose of 10B and 157Gd of 22.4 μg 10B / g cell and 17.818 μg 157Gd / g cell. It’s close to the cancer dose 25~30 μg 10B / g cell predicted in the literature. Our experimental results show that the 10B and 157Gd containing carbon nanoparticles are very effective, upon irradiation of neutron, in killing cells with high death fraction of 59 + 5 % and 75 + 5 % , respectively, for 10B and 157Gd containing carbon nanoparticles. Our experimental results are proofs of the concept that carbon nanoparticle can be used as neutron capture reagent.
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