| Summary: | 碩士 === 國立中山大學 === 生物醫學科學研究所 === 94 === In this study, we collected 18 plasma samples from NPC patients, and 10 plasma samples
from healthy person for plasma proteomic analysis. We classified these samples into 3 groups:
treatment, pretreatment and recurrent. Two-dimensional electrophoresis (2-DE) and
MALDI-TOF were performed followed by comparative and statistic analysis. In conclusions,
we totally identified 30 proteins in Nasopharyngeal carcinoma (NPC) plasma, and found 10
proteins with expression level down regulated (p<0.001). These proteins were characterized to
be Serotransferrin, Vitamin D-binding protein (VDB), alpha-1 antitrypsin (AAT), Haptoglobin,
Apolipoprotein B fragment, Syntaxin-7, Apolipoprotein, A-I, PRO1779, Transthyrin, MDN1
protein, respectively. Consequently, we established a protocol to remove high abundant
proteins (e.g., albumin, immunoglobin etc.) in plasma. We are especially interested in ATT
and VDB. Western blotting assay was performed to confirm ATT and VDB expression.
Furthermore, the quantity of ATT and VDB were measured by ELISA to obtain the threshold
value of these proteins. Finally, we want to realize the relationship between these down
regulation proteins and clinical parameters in NPC malignancy and tumor progression. Since
there are few protein expression research of NPC in clinical studies, our works will provide
insights in NPC studies for tumor progression with potential to elevate treatment efficiency.
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