| Summary: | 碩士 === 國立屏東科技大學 === 獸醫學系 === 94 === Canine distemper virus（CDV）causes generalized infection in dogs with prominent respiratory, gastrointestinal and nervous signs. Not only dogs, CDV can infect other carnivores, non-carnivores, ferret, mink, and marine mammals. CDV is classified into the Morbillivirus genus of the family Paramyxoviridae. CDV is an enveloped virus with single-stranded, non-segmented, and negative-sense RNA. It is a highly contagiously pathogen distributed worldwide. To dates, this disease has been controlled by vaccination with an attenuated live virus. Although an extensive vaccination program has been implemented, sporadic cases still were reported. Moreover, typical CDV can often been seen in those vaccinated dogs. The nucleocapsid protein（N protein）is a good candidate for production of effective vaccines. Therefore, the objective of this study is to produce N proteins using genetic engineering. The N gene of CDV was amplified using reverse transcription-polymerase chain reaction（RT-PCR）, and then confirmed with DNA sequencing（GenBank No. DQ435615, DQ522030）. The correct N gene was cloned into the expressed vector pET32a, transformed into BL21 Star（DE3）cells, and induced to express recombinant proteins using IPTG. After induction, the recombinant protein with correct molecular weights of 78 kDa was obtained. Subsequently, the specificity of this protein was verified using Western blots.
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