| Summary: | 碩士 === 國立屏東科技大學 === 水產養殖系 === 94 === To further understand the relationship between temperature and the sexual differentiation of hermaphrodite fish, this study aimed to different temperature on gonadal development, sex change and the expression of reproductive genes（ERα, ERβ, ARβ and Dmrt1）in protandrous black porgy, Acanthopagrus schlegeli. All fish are functional males for the first 2 years of life, but begin to change sex during the third year. In order to investigate the effects of temperature, black porgy were reared at 33℃ environment in advance and divided into 18, 23, 28 and 33℃ at pre-spawning season, respectively. The female ratio of black porgy were 5.55 %, 5.26 %, 25 % and 0 % after 45 days treatment, respectively. High-temperature（33℃）treatment during the spawning season could suppress gonadal development and serum LH concentration in the 1-2 years old males and block the natural sex change of 3-yr-old black porgy. During the non-spawning season, only the group of 18℃ treatment could stimulate level of serum LH, but there were no significant difference in the genes expression.
Finally, our findings confirm high water temperature could influence the reproductive physiology of black porgy. It also demonstrated for the first time that the warm water such as thermal discharge from nuclear power station could effect on gonadal development of hermaphrodite fish. In any case, temperature effects were implicated in the reproductive biology of fish.
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