Determination of Sulfonamide Residues in honey by High-performance Liquid Chromatography on-line with Solid-phase Extraction Device Packed with Molecularly Imprinted Polymer

• Main Authors: SUNG. MEI CHEN, 宋美貞
• Other Authors: 楊慶成
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/65912125415243481713

碩士 === 國立高雄師範大學 === 化學系 === 94 === Abstract This research is mainly used molecularly imprinted polymers as the stationary phase of the solid-phase extraction column,and on-lined with HPLC analysis of the content of 17 sulfonamides determinative used by 『the directory of animaled physic of bureau of animal and plant health inspection and quarantine council of agriculture, executive yuan』. The study investigate （1）the optimal conditions of HPLC to simultaneous analyze 17 sulfonamides,（2）the optimal conditions of on-line MIP/SPE, including the sample injection volume, the time of desorption, and the concentration of the eluention solvent. By the optimal conditions, the linear range, consistency, and detection limit were studied by both of the two on-line methods. And the real honey samples were examined by the proposed methods. The optimal conditions of on-lined MIP/SPE extraction is injection volume 800 μl, the pH of the sample solution is 4.7, the MIP/SPE extraction column, the flow rate of 1.0 ml/min of gradient elution of formic acid aqueous solution/acetonitrile, the temperature is 28℃, and the detection wavelength set at 270 nm. The honey sample spiked with 17 sulfonamides were investigated by the optimal conditions of the MIP/SPE on-line methods.The recoveries were 76.01～115.07％ ( except STZ、SMI、SDM ), the detection limit is 0.02 ppb.