| Summary: | 碩士 === 國立高雄第一科技大學 === 環境與安全衛生工程所 === 94 === ABSTRACT
In order to find the characteristics of co-culture of hydrogen-producing
predominate, Clostridium beijerinckii, Clostridium butyricum, and Bacillus
thermoamylovorans were subsequently performed according to a central composite
design and a mixture design.
Experimental results indicated that co-culture of the C. beijerinckii and C.
butyricum could convert waste yeast into hydrogen, and B. themoamylovorans was hard
to use the organics converting to hydrogen alone. However, the B.themoamylovorans
plays an important role on the enhancement of C. beijerinckii and C. butyricum
producing hydrogen. That was because the B.themoamylovorans could stimulate
hydrogen-producing potential and specific hydrogen production rate, and shorten the lag
of their co-culture. A hydrogen-producing yield of 3.34 (ml H2/g COD added waste
yeast) from a 100-ml co-culture experiment could be found when 14.4 ml of
C.beijerinckii (6.5 × 106 cuf) was mixed with 14.4 ml of B.thermoamylovorans
(9.8 × 106 cuf). In addition, its main by-product of volatile fatty acid was butyric acid.
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