| Summary: | 碩士 === 國立東華大學 === 生物技術研究所 === 94 === Because liver associated diseases have been the top-ten major causes of death in Taiwan recently, the medical research in liver regeneration and hepatic differentiation of stem cells become very important. The main functions of hepatocyte growth factor (HGF) include stimulating the DNA synthesis of DNA in hepatocyte, and promoting growth and differentiation of hepatocyte. The overall objective of this study is to investigate the effect of introducing the recombinant viral vectors carrying rhHGF gene into human bone marrow-derived mesenchymal stem cells (BMMSC) on their hepatic differentiation. Recombinant viruses including AAV-rhHGF virus and lenti-rhHGF-IRES-GFP virus were produced by cotransfection and recovered by CsCl centrifugation. The infectivities and bioactivities of the recombinant viruses obtained were then confirmed. Based on the experimental tests on the typical liver-related characteristics including cell morphology, gene expression profile, immunostaining, uptake of low density lipoprotein and the metabolism of CYP450, it was found the hepatic differentiation of BMMSC could be facilitated by the infection of recombinant lentivirus with encoded gene of HGF. Hepatocyte-like cells could be derived from BMMSC after two days’ infection of lenti-rhHGF-IRES-GFP virus.
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