The Study to Evaluate the Relationship between X Chromosomes and the Occurrence of Lung Cancer in Taiwanese Female Population

• Main Authors: Yeh En-Hsuan, 葉恩瑄
• Other Authors: Chien Yi-Chih
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/68477624519050428884

碩士 === 國立彰化師範大學 === 生物學系 === 94 === Abstract The purpose of present study was using nonrandom X - chromosome inactivation patterns to analyze the relationship between X chromosomes and the occurrence of lung cancer in Taiwanese female population. In addition, LOH analysis on X chromosome was performed to look for the possible lung cancer-specific locus of tumor suppressor gene(s) on X chromosome. We found that among female patients with lung cancers, hepatocellular carcinoma, gastric cancers, and colorectal cancers revealed no difference in distribution of the X - chromosome inactivation patterns in tumor tissues ( p = 0.413 ). There was no direct correlation of nonrandom inactivation pattern with the tumor formation of female patients of lung cancers. However, there was a significant correlation of inactivation pattern with the tumor size of female patients of lung cancers ( p = 0.036 ). If the tumor tissues were with random inactivation pattern, their tumor size were large ( > 25 cm3 ) ; whereas those with nonrandom inactivation pattern were small ( ≦ 25 cm3 ). The frequency of LOH of both DXS1226 and DXS1060 microsatellite markers in female lung cancer patients were higher than that in female patients with hepatocellular carcinoma, gastric cancer, and colorectal cancer, but no significant difference was found in statistical analysis. The results suggested that both DXS1226 and DXS1060 might be possible specific biomarkers in detecting the lung cancers of Taiwan female population. We found that the presence of LOH of DXS8059 ( p = 0.02 ) and DXS1047 ( p = 0.083 ) in female lung cancers was associated with tumor sizes. In addition, the lung patients had LOH on both DXS8059 and DXS1047 were prone to tumors with nonrandom X chromosome inactivation pattern ( p = 0.083 ), especially in DXS1047 ( p = 0.055 ). There was a strong correlation between tumor stages and the frequency of LOH of DXS8067 ( p = 0.037 ). Patients with late tumor stages ( stages 3 and 4 ) tended to have LOH at DXS8067. We also demonstrated that the regions with high frequency of LOH in female patients with lung cancers and breast cancers were different, suggesting that more than one putative TSGs on X chromosome. Allelic patterns of DXS8059 in Taiwanese women ( n = 120 ) were analyzed. To our surprise, the allelic patterns between the two female groups, healthy individuals and cancer patients, were distinct ( p < 0.0001 ). Our findings demonstrated that female, whose allelic patterns of DXS8059 were homo-209 bp or both non-211 bp, had higher risks to suffer lung cancers, breast cancers, hepatocellular carcinoma, or gastric cancer. As a conclusion, we have demonstrated that there was no direct correlation of nonrandom X-chromosome inactivation patterns with the tumor formation of female patients of lung cancer, but there was significant correlation with the development of tumor tissues of lung cancers. Due to different regions of high frequencies of LOH on X chromosomes occurred in female patients with lung cancers and breast cancers, we suggested that there be more than one putative TSGs on X chromosomes. We have assessed that DXS8059 allele patterns might be an useful and convenient biomarker for detection of females, who have higher risks to suffer cancers.