| Summary: | 碩士 === 國立中央大學 === 環境工程研究所 === 94 === The microbial community structure analysis of a full-scale Barnard process for treating photoelectrical industry wastewater was investigated in this study. The group-specific 16S rRNA primers and PCR-DGGE method were applied to figure out the profile of the microbial communities and the connection to the processes. Experimental results indicate that with different group-specific 16S rRNA primers including bacteria, kingdom acidobacterium, α-proteobacteria, β-proteobacteria ammonia oxidizers, actinomycetes, and methylotrophs, coordinate to PCR-DGGE method, can facilitate the classification of dominant species before clone library establish and promote the sensitivity of the PCR-DGGE method. In addition, the results of PCR-DGGE analyzing can be a good reference for the clone library design which enhances the efficiency of the cloning for the followed experiments. Suspending sludge samples for this study were collected from a full-scale Barnard process by treating the photoelectrical industry wastewater. The process is constituted by an anoxic tank-aerobic tank-anoxic tank-aerobic tank treatment schedule. The 16S rRNA clone library was established by PCR product from bacteria and β-proteobacteria ammonia oxidizers, which including 11 bacteria and 8 β-proteobacteria ammonia oxidizers clones. Microbial community analysis result shown that the β-proteobacteria is the dominant species in all the Barnard process biological processes. Furthermore, in the clone library, 18 clones belonged to β-proteobacteria and 6 of them demonstrate similarity to Oxalobacteraceae. Janthinobacterium sp. is the mainly dominant species and distributed more than 50% of bar weight to the total population. Nitrosospira sp. is also a dominant species in the β-proteobacteria ammonia oxidizers with distribution of more than 30% of the total population in the system. The bacterial 16S rRNA gene sequences of clone B3, N2, and N5 shown less than 97% similarity to the known strain in the database of NCBI. Accordingly, the clone B3, N2, and N5 obtain the probability for belong to the novel discovery strains which found by this study.
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