Over-expressing ES cell-specific transcription factors suppresses the differentiation of myoblasts

• Main Authors: Yu-Jung Chang, 張毓容
• Other Authors: Shen-Liang Chen
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/xbaj2w

碩士 === 國立中央大學 === 生命科學研究所 === 94 === The potential of cell mediated tissue regeneration is hampered by both immune rejections of engrafted allogenic ES cells and the difficulties of isolating and propagating autologous stem cells. However, if the somatic cells can be induced to transdifferentiate into precursors of target cell types in vitro, cell mediated tissue regeneration will be more applicable. In this study, we introduced two ES cell-specific transcription factors, Nanog and Oct3, into sol8 myoblast using retrovirus system and examined the effects on differentiation stage of the stable clones. Over expressing Nanog and Oct3 simultaneously, but not independently, impaired the terminal differentiation of Sol8 myoblasts. Furthermore, the expression of myogenic regulator factors (MRFs), MyoD and Myogenin, is abolished in Sol8-Nanog/Oct3 cells. In addition, the slightly repression of Myf5 expression could be further diminished after β-mercaptoethanol treatment. Our data suggest that although over expressing Nanog and Oct3 in myoblasts can surpress myogenic differentiation, whether those cells acquired pluripotency still needs to be further investigated.