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• Main Authors: Chih-Kang Peng, 彭志剛
• Other Authors: Shin-Shing Shyu
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/86170708734254434881

博士 === 國立中央大學 === 化學工程與材料工程研究所 === 94 === Abstract The extracellular matrices (ECMs) of hard tissue are mainly composed of organic and inorganic such as collagen, glycosaminoglycans (GAGs) and hydroxyapatite (HA). This study proposed a method to produce size-controlled nanoapatites that are mineralized chitosan/gelatin/tripolyphosphate (TPP) nanocomposites for the use as biomimetic bone tissue-engineering materials. The chitosan/gelatin/TPP ternary composites demonstrated nano-sized phase separation as observed from the atomic force microscopy (AFM) study. FT-IR, X-ray diffraction (XRD), tensile strength and dynamic mechanical analysis (DMA) showed that TPP polyion played an important role in affecting the chemical structures, and in dominating the crystallization and mechanical properties of the chitosan/gelatin/TPP nanocomposites. The chitosan/gelatin/TPP nanocomposite films were used as biomimetic templates for nucleation and growth of hydroxyapatite crystals on the films from simulated body fluid (SBF). The spherical aggregates of hydroxyapatite on the film surface were identified with X-ray diffraction and energy-dispersive X-ray (EDS). The results suggested that chitosan/gelatin/TPP nanocomposites are promising materials for biomineralization of hydroxyapatite. Chitosan/gelatin/TPP ternary composite scaffolds were prepared by a freeze-drying technique, and could also form mineralized apatite on their pore wall surface. The biomimetic organic/inorganic nanocomposite scaffold is now under examination of its osteoconductivity. Finally, from a simple osteoblastic cell (ROS 17/2.8) in vitro evaluation, we found that the film is non-toxic to cell. Furthermore, through the ALP stain and activity tests of alkaline phosphatease, we could get that the film treated by biomineralization has a property of osteoconductivity. This property would be apparent with the increase of hydroxypatite in the film. As we studied above, we could find that the biomineralization materials of chitosan/gelatin/tripolyphosphate acquired in this study has its utilities in biomimetic bone tissue-engineering.