| Summary: | 碩士 === 國立交通大學 === 生物資訊研究所 === 94 === RNA editing is the modification of RNA sequence through nucleotide deletion, insertion, or base modification mechanisms. RNA editing mechanisms affect the pre-mRNA splicing, frameshfits, RNA structure changes and mRNA translation. Recently, two types of RNA editing such as A-to-I and C-to-U were investigated. A database comprising RNA editing sites in mammalian genomes is crucial for deciphering the RNA editing mechanism. In this thesis, we established a database which comprises both the experimentally validated RNA editing sites and computationally predicted RNA editing sites. Experimentally validated sites ware obtained by literature survey. A computational method based on sequence comparing analysis is implemented for identifying putative RNA editing sites. Additionally, cross-species comparison is performed to confer the evolution meanings of the RNA editing site. Motif discovery method is applied to discover RNA structural motifs, which are potentially related to each type of editing site. The database is now available in http://EdRNA.mbc.nctu.edu.tw/.
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