| Summary: | 碩士 === 國立暨南國際大學 === 應用化學系 === 94 === Kojic acid is known to be able to suppress the generation of melanin effectively,
because kojic acid can bind with the copper ion located in the active site of
tyrosinas which is a key enzyme responsible for melanin synthesis. Forskolin can
induce the melanin production in melanoma cells via the activation of adenylate
cyclase to increase intracellular cyclic AMP concentration.
We first investigated the effect of kojic acid treatment on the forskolin (100
μM)-induced melanin production in melanoma cells (B16-F10). When 10mM of
kojic acid was employed, the generation of melanin can be effectively suppressed.
This amount of kojic acid can control up to about 79% of the synthesis of melanin.
We then studied the effect of kojic acid treatment on the endogenous melanin
production in the melanoma cells. The results indicated that 10mM of Kojic Acid
can control about 68% of the synthesis of melanin.
Next, in order to findout the intracellular concentration of kojic acid following
the extracellular treatment, the cells were lysed and the concentration of kojic acid
was analyzed by HPLC. Unfortunately, we were not able to observe detectable
amount of kojic acid in the cells. The reason might be that the intracellular
concentration of kojic acid is too low probably due to its strong binding with
tyrosnase.
Finally, L-ascorbic Acid was added to observe its efficiency in suppressing
melanin. L-ascorbic Acid can be oxidized easily; thus, there appeared to be no
apparent result after three days of cultivation. These series of data along with in
tube experiment can be an effective comparison mechanism to test the efficiency of
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whitening medicine in the future.
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