| Summary: | 碩士 === 國立成功大學 === 製造工程研究所碩博士班 === 94 === This study aims at investigating the optimal condition for HA coating in vitro by biomimetic immersion. In order to study the importance of the factors for deposited calcium phosphate apatite, it selected soaking temperature, immersing solutions, and the surface structure of Titanium dioxide, individually. The surface treatments of Ti6Al4V samples was carried out by passivating with heated in 400°C air (HT) and immersing in volume 34% nitric acid (HP) first .Then immersed them in simulated body fluid (SBF) or supersaturated calcification solution (SCS) at 10°C, 37°C or 80°C. The surface roughness and morphology is measured by Scanning Probe Microscope (SPM); the chemical property of surface deposition is analyzed by Electron Spectroscopy for Chemical Analysis (ESCA) and Energy Dispersive Spectrometer (EDS); the crystal of surface deposition is observed by Multipurpose X-Ray Thin-Film Diffractometer (TF-XRD); images of the calcium phosphate is taken by High Resolution Field-Emission Scanning Electron Microscopy (FE-SEM). Estimated the biocompatibility of the optinal immersed condition by using
MTT, alkaline phosphatase(ALP) ,and FE-SEM to observed the morphology of osteoblast
By using SPM and FE-SEM, it has been observed that increasing the soaking temperature would raise the surface roughness and the covered area of apatite. With ESCA analyzing, immersed at higher soaking temperature would increase the production of calcium phosphate apatite. In addition, the structure mixed by anatase and rutile had higher roughness than the pure structure of rutile. This study weighed the importance of factors and provided a simple and easily controllable optimal condition for biomimetic immersion.
|
|---|
