Development of Action Potential and Impedance Measurement System for Cultured Neurons on Multielectrode Array

• Main Authors: Shao-Hsin Yeh, 葉紹興
• Other Authors: Jia-Jin Chen
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/02249377849587889917

碩士 === 國立成功大學 === 醫學工程研究所碩博士班 === 94 === Recent electrophysiology development of multielectrode array (MEA) allows researchers to perform extracellular stimulation and sensing of patterned neural network cultured on a surface-modified substrate. The aims of this study were to construct an action potential (AP) and impedance measurement system for neurons cultured on multielectrode array (MEA) to validate the extracellular stimulation and recording for observing the electrophysiological signal transmission in neuronal network. An extracellular stimulator with negative feedback to produce the biphasic micro-current pulse for neuron stimulation was built in this study. In verifying of the sealing resistance and shunt capacitance between the neurons and electrode, experiments were performed by impedance spectra and long-term impedance monitoring for neurons or cells seeded and grew on multielectrode. In the long-term impedance measurement, we used the high osmosis PBS to simulate damage in neurons in vitro. For extracellular stimulation study, neuronal activity of differenced PC12 cells successful was recorded and the suitable stimulation window was determined. However, the stimulation results were affected by electrode impedance as well as sealing impedacen resulting from neuron cells covering the microelectrode. Further development of microcontact printing for patterned neuron network cultured on the MEA should provide an novel platform for in-vitro neuronal outgrowth under varied conditions via impedance and electrophysiological measurements.