Study on enterovirus 71 neuroinvasion through disruption of blood-brain barrier

• Main Authors: Shin-Chao Lin, 林士超
• Other Authors: Chun-Keung Yu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/66461612923578195427

碩士 === 國立成功大學 === 微生物及免疫學研究所 === 94 === Previous study in our laboratory demonstrated that enterovirus 71 (EV71) possesses strong neurotropism and that EV71 might transmit with fast axonal transport in neuron cells in mice. However, whether EV71 could invade the central nervous system through circulation by alterating the permeability of blood-brain barrier (BBB) is not known. In this study, we used Evans blue dye, which would form an insoluble complex after binding with serum albumin to evaluate the permeability of BBB after EV71 infection. Our results showed there was a viremia as early as 24 hours after oral inoculation of EV71 without detectable infectious viruses in brain tissues. The BBB permeability was increased at 30, 60, and 72 hours post-inoculation. Coincidentally, a small amount of viruses could be detected in medulla at 30 hours post-inoculation without significantly increase followed time. EV71 was first detected in lumbar and thoracic sections of the spinal cord at 48 hours post-inoculation with a gradual increase in titer from the lower to the upper sections. Viruses appeared in cervical section of the spinal cord at 72 hours post-inoculation. EV71 infection induced an increase in TNF-alpha and IL-10 systemically and locally in brain tissues. Collectively, retrograde axonal transport as well as viremia may responsible for the transport of EV71 to the brain.