| Summary: | 碩士 === 國立成功大學 === 航空太空工程學系碩博士班 === 94 === The micro flow cytometry is an important technology for sample analysis in bio-chip science. The advantage is that bio-particles can be analyze rapidly no matter cell size or type. Among various methods, recently, the development of physical methods (without external forces) by making use of the laminar flow profile inside microchannels has drawn certain attention. In this study, we intend to integrate the above methods in our systems for separation of particles in nature flow and be used for analysis in the future.
First, we introduce the theories of micro-hydrodynamic focusing and particle-separation method, then prove the suitable flow resistance equation and explain the design of micro-fluidic chip. The study employed MEMS fabrication technique for building microchannel using PDMS elastomer. The master is formed on silicon wafer using an epoxy-based photoresist. PDMS is cast against the master producing molded layer containing channel then bonded on a glass to build microchannel.
Theoretically, by controling the flow rate at the inlet, particles will move along centerline of the channel. When particles flow through the obstacle, they follow different paths according to their sizes due to laminar flow profiles. In this study, we can separate 2 μm and 10 μm diameter particles in an integrated microfluidic chip.
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