Immobilization of chitosan on the plasma-activated poly-L-lactic acid film surface using evaporated acrylic acid as the intermediate

• Main Authors: Hsin-Hung Chen, 陳信宏
• Other Authors: Jiunn-Der Liao
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/65502824103928171400

碩士 === 國立成功大學 === 材料科學及工程學系碩博士班 === 94 === Poly-L-lactic acid ( PLLA ) has biodegradable , biocompatible properties and suitable mechanical character, so it can be manufactured as scaffolds for nerve conduit. Chitosan with cell affinity is used to enhance the regeneration of peripheral nerves. The study is using radicals produced on the plasma-activated PLLA film combining evaporated acrylic acid（AAc）monomers and then immobilizing chitosan on the PLLA film by amide bonding formed between carboxyl and amine group. We expect that it can combine both advantages between biodegradability of PLLA and biological functionality of chitosan. The surface morphology is observed by scanning electron microscope. The respective chemical structure change following sequential reactions were identified using Fourier transform infrared spectrometer-attenuated total reflection and X-ray photoelectron spectroscopy. Then we discover the biological functionality by antibacterial and cell attachment tests. The results demonstrated that evaporated AAc monomers could be grafted on PLLA film and chitosan could also be immobilized on AAc-grafted PLLA film by amide bonding. The morphology of most fibroblasts cultured on the chitosan-immobilized PLLA film maintained round after 48 hours culturing. We infer that chitosan-immobilized PLLA film would reduce the attachment of fibroblasts on the surface, and therefore the proliferation rate of fibroblasts would also be repressed. The results can be applied on the manufacturing of scaffolds for nerve conduit that can decrease the appearance of scar tissues and enhance the efficacy of peripheral nerve regeneration.