| Summary: | 碩士 === 國立成功大學 === 生理學研究所 === 94 === The female steroidal hormone estrogen triggers a wide variety of biological effects in a number of target systems. The effects of estrogen are mediated by 2 different nuclear hormone receptors, ERα and ERβ.
Previous study reported that estrogen play an important role in ERβ dominant systems, heart and lung. But the effects of ERβ on heart and lung are ont all clear. Proteomics approaches provide a large scale analysis of ERβ-mediated proteins in cardiopulmonary system.
In this study, we use the myocardial infarction model and ultra fine carbon black (ufCB)-induced lung inflammation model. Estrogen receptor beta agonists, DPN and genistein, are applied to analyze the effect of ERβ on this two models. And 1-D gel combined with quantitative LC/MS/MS is applied to analyze the estrogen receptor beta mediated proteins alteration in the two models. In the result, quantitative LC/MS/MS reveals that estrogen receptor β agonist decrease energy metabolism proteins expression in this two models. This study provides a new viewpoint. Estrogen receptor β may repress the upraised energy metabolism after stress in heart and lung.
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