Reprogramming the adult skin fibroblast cells with embryonic stem cell extract

• Main Authors: Ching-Hsun Kang, 康景勛
• Other Authors: Chein Tai
• Format: Others
• Language: en_US
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/26636879067512663805

碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 94 === 　Nuclear reprogramming is defined as altering the gene activity of differentiated cells to express the characteristics and function of different lineage, even resume their pluripotency. Several researches have demonstrated that nuclear reprogramming is possible. Nuclear transfer with somatic cells had proven to be able to give rise to cloning animals and derivation of embryonic stem cells. Induction of plasticity of adult stem cells could promote somatic cells to trans-differentiate into other cell lineages. 　After increasing permeability of the plasma membrane and introduction of ES-D3 cell extract, the morphology of somatic cells changed into a ES cell-like shape. Quantitative real-time PCR analysis showed both reprogrammed Dunni cells and HS-68 cells expressed Oct-4, Nanog and Pecam-1 genes which are stage-specific to undifferentiated ES cells. Strong alkaline phosphatase (AP) activity was also observed in reprogrammed cells. Under spontaneous differentiation, reprogrammed somatic cells could trans-differentiate into different lineages of three primary germ layers. According to our results, terminal differentiated human and mouse fibroblasts can be reprogrammed by mouse embryonic stem cell extracts. However, the protocol should be further modified to achieve a better efficiency on reprogramming. 　In the present study, we found that the cellular characteristics of somatic fibroblast cells derived from mouse skin (Dunni cells) and human foreskin (HS-68) could be reprogrammed by the cell extract of mouse embryonic stem cells (ES-D3).