The Preparation of Fe3O4 Nanoparticles and their Applications in Biomedicine

• Main Authors: Fong-Yu Cheng, 鄭豐裕
• Other Authors: Chen-Sheng Yeh
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/55536902545845859091

博士 === 國立成功大學 === 化學系碩博士班 === 94 === By modifying synthesized method to prepare water-soluble and biocompatible Fe3O4 nanoparticles which no polymer coated is a new technology. The as-synthesized Fe3O4 nanoparticles not only have above properties but also provide functional groups to achieve derivation. This method simplified some condictions of reaction, and it reduced some problems in synthesized methods which developed before. The Fe3O4 nanoparticles can apply to different fields by modifying the surface of them. According to diverse surface modifications of the Fe3O4 nanoparticles, their suited applications such as biomedicine, analysis, and separation are different. The Fe3O4 nanoparticles also have no limits required by biomedicine and provide many new ideas for developments and reasearchs. They can combine with most biomolecules to become a new-type nanomaterial, developing more powerful technologies. There are three types of Fe3O4 nanoparticles prepared in this article. First, ammonium-type Fe3O4 nanoparticles can control the behaviors of DNA absorption and desorption by adjusting pH values. This reversible process can set up a simple model that applies to drug delicery systems. Second, carboxyl acid-type Fe3O4 nanoparticles could successfully separate genomic DNA in a complex condition of human whole blood. This separation technology of magnetic nanoparticles affords a rapid, easy, and efficient method in detecting diseases. The final type is amino-type Fe3O4 nanoparticles. They can achieve many applications, enzyme immobilization, mass-spectrum analysis, DNA probe, polymerase chain reaction (PCR), protein separation and contrast agent, combined with other molecules. Amino-type Fe3O4 nanoparticles is more important type than two types because of its wide variations in modifying functional groups on the surface of nanoparticles. With different functional groups, they can be applied to specifiec fields which needed. In this article, arrording to different chatacterastics of functional groups, the three different types of Fe3O4 nanoparticles could be applied in various fields. Their detail or special properties could be understanded with experimental results and a serious of tests. With the advantages of combining chemical molecules and biomolecules, the applications of Fe3O4 nanoparticles extend and develop more powerful and new uses in biomedicine field, including separation, diagnosis, detection and image. The as-synthesized Fe3O4 nanoparticles in this modified method really show its advantages, new technologies, stability, and variable applications in many fields.