Constituents of Phaius mishmensis

• Main Authors: Chen-Wei Jao, 饒真瑋
• Other Authors: Pei-Lin Wu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/05327065727583807065

碩士 === 國立成功大學 === 化學系碩博士班 === 94 === 　Phaius mishmensis belongs to Orchidaceae. According to the preliminary screening result by National Health Research Institutes, we found that P. mishmensis is a valuable material to investigate the anticancer active principles. The methanolic extract of dry Phaius mishmensis (3.5 kg) was suspended in water then partitioned with n-hexane, chloroform, and ethyl acetate successively. After extensive column chromatography and thin-layer chromatography, 17 new compounds, 2-methoxycarbonylindolin-3-one (3), phaindirubinol (5), phaindolanthranilate (7), 1H,3H-quinazoline-2,4-dione (8), 3-(2'-hydroxyphenyl)-3H-quinazolin-4-one (9), phaitanthrin-A (11), phaitanthrin-B (12), phaitanthrin-C (13), phaitanthrin-D (14), phaitanthrin-E (15), phainanthrene-A (17), (7'R,8'R)-phainanthrene-B (18), methyl hematinate (20), 2-aminobenzonitrile (21), 2-(N-methoxycarbonylamino)benzamide (22), 3-hydroxy-4,5-dimethoxypropiophenone (24), and methyl (S)-7-hydroxydihydroferulate (34), as well as 30 known compounds, isatin (1), 3,3-dimethoxyisatin (2), indirubin (4), 3-methoxycarbonylindole (6), tryptanthrin (10), candidine (16), 3-ethyl-4-methylpyrrole-2,5-dione (19), methyl anthranilate (23), benzoic acid (25), 4-hydroxybenzaldehyde (26), methyl 4-hydroxybenzoate (27), 4-oxyacetophenon (28), vanillaldehyde (29), vanillic acid (30), methyl vanillate (31), syringaldehyde (32), 2-methyl-4-nitrophenol (33), dihydrocinnamic acid (35), p-dihydrocoumaric acid (36), methyl p-dihydrocoumarate (37), ficusol (38), cinnamic acid (39), trans-p-coumaric acid (40), methyl trans-p-courmarate (41), ferulic acid (42), methyl ferulate (43), methyl cis-p-courmarate (44), 3-oxo-α-ionol (45), dehydrovamifoliol (46), and methyl hydrogen succinate (47) were isolated. 　Among them, trypthanthrin (10) showed strong cytotoxic activity against MCF-7, NCI-H460, and SF-268 cell lines and phaitanthrin-B (12) also showed strong cytotoxicity against MCF-7 and NCI-H460 cell lines.